• Synmatrix-O

  Defined Organoid Culture Matrix (Matrigel Alternative)

  A fully defined, ECM-mimetic photocrosslinkable hydrogel matrix designed to replace undefined basement membrane extracts (e.g. Matrigel) in organoid expansion, passaging, and advanced 3D in vitro modelling.

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  Key Features

  Defined composition: eliminates batch variability associated with animal-derived matrices.

  ECM-mimetic environment: supports 3D organoid growth with physiologically relevant structure.

  Photocrosslinkable control: enables precise tuning of stiffness and architecture.

  Reproducible workflows: supports standardised and scalable organoid culture.

  Application-oriented: compatible with downstream assays, screening, and engineered systems.

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  Typical Applications

  Organoid culture, expansion, and passaging as a defined alternative to Matrigel

  Long-term organoid studies with improved reproducibility

  Patient-derived organoid (PDO) models

  3D disease modelling

  Drug response and screening

  Integration with organ-on-chip and microphysiological systems

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  Specifications

  Property	Details
  Product name	Synmatrix-O
  Product type	Defined ECM-mimetic photocrosslinkable hydrogel
  Form	Solid (lyophilized / sponge-like)
  Appearance	White to off-white
  Application	Organoid culture and 3D systems
  Crosslinking	Photoinitiated
  Wavelength	365–405 nm
  Preparation	Dissolution in aqueous buffer
  Storage	Frozen recommended

Validated Systems

Synmatrix-O is designed for organoid-based 3D culture systems, including stem cell-derived organoids, patient-derived organoids, and engineered 3D disease models.

Preparation & Use

1. Photoinitiator preparation: prepare sterile LAP solution (0.2% w/v), filter sterilize, store at 4 °C protected from light.

2. Hydrogel preparation: dissolve Synmatrix-O with LAP solution and DPBS to 6% (w/v), stir under light protection until clear.

3. Gel formation: mix with organoids or cells, dispense, and crosslink under 405 nm light (~30 s).

4. Culture setup: wash with DPBS, replace with culture medium, incubate.

Safety

Use appropriate PPE. Avoid inhalation, ingestion, and contact with skin or eyes. Protect from light. Dispose according to local regulations. For research use only.

What’s Included

Synmatrix-O hydrogel precursor

Quick-start protocol